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CD Genomics offers highly flexible shotgun library construction services, including shotgun sequencing of the completed library. High quality random-sheared shotgun libraries of BAC clones and whole genomes are regularly produced at CD Genomics. Our shotgun library construction has been done with a variety of vectors such as plasmid, cosmid, fosmid, BAC, and YAC.
• Plasmid DNA purification and/or quality analysis of in-coming DNA
• Random shearing to produce a population of DNA fragments
• Ligation into a standard cloning vector and transformation
• Libraries submitted to customer as plates, glycerol stocks, or purified DNA
• QC by PCR or DNA sequencing