5

year

Shotgun Library Construction Service

Shotgun Library Construction Service
group name
Pharmaceutical Chemicals
Min Order
1 gram
brand name
CD Genomics
payment method
update time
Mon, 26 Jan 2015 00:52:41 GMT

Packging & Delivery

  • Min Order1 gram

Briefing

CD Genomics offers highly flexible shotgun library construction services.

Detailed

CD Genomics offers highly flexible shotgun  library construction services, including shotgun sequencing of the completed  library. High quality random-sheared shotgun libraries of BAC clones and whole  genomes are regularly produced at CD Genomics. Our shotgun library  construction has been done with a variety of vectors such as plasmid, cosmid, fosmid, BAC,  and YAC.

Construction Steps:

  • Plasmid DNA purification and/or quality analysis of in-coming DNA

  • Random shearing to produce a population of DNA fragments

  • Ligation into a standard cloning vector and transformation

  • Libraries submitted to customer as plates, glycerol stocks, or purified DNA

  • QC by PCR or DNA sequencing